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Identification and analysis of MHC class II DRB1 (Patr-DRB1) alleles in chimpanzees
Article first published online: 26 JAN 2006
DOI: 10.1111/j.1399-0039.2006.00539.x
Additional Information
How to Cite
Bak, E. J., Ishii, Y., Omatsu, T., Kyuwa, S., Tetsuya, T., Hayasaka, I. and Yoshikawa, Y. (2006), Identification and analysis of MHC class II DRB1 (Patr-DRB1) alleles in chimpanzees. Tissue Antigens, 67: 134–142. doi: 10.1111/j.1399-0039.2006.00539.x
Publication History
- Issue published online: 26 JAN 2006
- Article first published online: 26 JAN 2006
- Received 19 August 2005; revised 5 October 2005; accepted 7 November 2005
- Abstract
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Keywords:
- allelic polymorphism;
- microsatellite repeat length variability;
- Patr-DRB1 allele;
- PCR technique
Abstract
The MHC-DRB1 gene is known to display the most extensive allelic polymorphisms among MHC class II genes. We attempted the selective identification of chimpanzee (Pan troglodytes) DRB1 (Patr-DRB1) alleles using the polymerase chain reaction (PCR) technique in three steps: first, we performed Patr-DRB1*02 lineage-specific 8-kb PCR for *02 lineage detection in each chimpanzee; second, we performed 620-bp PCR for amplification of full-length exon 2; and finally, we carried out an insert check using the pattern of microsatellite repeat length variability. In the genomic DNA of 23 chimpanzees, nine Patr-DRB1 alleles containing two new alleles were detected. Our approach provides a relatively effective method of identifying Patr-DRB1 alleles in individual chimpanzees and should also contribute to our understanding of the features of MHC molecules in non-human primates.