Isolation, molecular cloning and in vitro expression of rhesus monkey (Macaca mulatta) prominin-1.s1 complementary DNA encoding a potential hematopoietic stem cell antigen

Authors

  • S. M. Husain,

    Corresponding author
    1. Division of Stem Cell Transplantation, Department of Hematology-Oncology, St. Jude Children’s Research Hospital, Memphis, TN 38105, USA
      S. Mazher Husain
      332 N. Lauderdale St. MS #321
      Memphis, TN 38122
      USA
      Tel: 1-901-458-4636
      Fax: 1-901-495-4023
      e-mail: syed.husain@stjude.org or smazher72@yahoo.com
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  • Y. Shou,

    1. Division of Experimental Hematology, Department of Hematology-Oncology, St. Jude Children’s Research Hospital, Memphis, TN 38105, USA
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  • B. P. Sorrentino,

    1. Division of Experimental Hematology, Department of Hematology-Oncology, St. Jude Children’s Research Hospital, Memphis, TN 38105, USA
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  • R. Handgretinger

    1. Division of Stem Cell Transplantation, Department of Hematology-Oncology, St. Jude Children’s Research Hospital, Memphis, TN 38105, USA
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S. Mazher Husain
332 N. Lauderdale St. MS #321
Memphis, TN 38122
USA
Tel: 1-901-458-4636
Fax: 1-901-495-4023
e-mail: syed.husain@stjude.org or smazher72@yahoo.com

Abstract

Human prominin-1 (CD133 or AC133) is an important cell surface marker used to isolate primitive hematopoietic stem cells. The commercially available antibody to human prominin-1 does not recognize rhesus prominin-1. Therefore, we isolated, cloned and characterized the complementary DNA (cDNA) of rhesus prominin-1 gene and determined its coding potential. Following the nomenclature of prominin family of genes, we named this cDNA as rhesus prominin-1.s1. The amino acid sequence data of the putative rhesus prominin-1.s1 could be used in designing antigenic peptides to raise antibodies for use in isolation of pure populations of rhesus prominin-1+ hematopoietic cells. To the best of our knowledge, there has been no previously published report about the isolation of a prominin-1 cDNA from rhesus monkey (Macaca mulatta).

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