14th International HLA and Immunogenetics Workshop: Report from the Sequencing-Based Typing Group


Dr M.G.J. Tilanus
Department of Immunology
F03.722, University Medical Centre Utrecht
Heidelberglaan 100
3584 CX Utrecht
The Netherlands
Tel: 31 (0)30 2517107
Fax: 31 (0)30 2544990
e-mail: M.Tilanus@umcutrecht.nl


Sequencing-Based Typing (SBT) provides the golden standard for the identification of polymorphism among the human leukocyte antigen (HLA) alleles. Several SBT approaches have been published. Updated protocols for HLA-DRB and -DQB were presented and an approach for DQA, covering all exons, validated. The highest level of allele typing can be realized with strategies for resolving allele and genotype ambiguities. Bioinformatics provides the tools for exchange and sharing data with a community standard in XML format.