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Distribution of the killer cell immunoglobulin-like receptors in Mexican Mestizos

Authors

  • G. Contreras,

    1. Department of Immunology & Immunogenetics, Instituto de Diagnóstico y Referencia Epidemiológicos, InDRE, Secretary of Health, Mexico City, Mexico
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  • C. Aláez,

    1. Department of Immunology & Immunogenetics, Instituto de Diagnóstico y Referencia Epidemiológicos, InDRE, Secretary of Health, Mexico City, Mexico
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  • A. Murguía,

    1. Department of Immunology & Immunogenetics, Instituto de Diagnóstico y Referencia Epidemiológicos, InDRE, Secretary of Health, Mexico City, Mexico
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  • D. García,

    1. Department of Immunology & Immunogenetics, Instituto de Diagnóstico y Referencia Epidemiológicos, InDRE, Secretary of Health, Mexico City, Mexico
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  • H. Flores,

    1. Department of Immunology & Immunogenetics, Instituto de Diagnóstico y Referencia Epidemiológicos, InDRE, Secretary of Health, Mexico City, Mexico
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  • C. Gorodezky

    Corresponding author
    1. Department of Immunology & Immunogenetics, Instituto de Diagnóstico y Referencia Epidemiológicos, InDRE, Secretary of Health, Mexico City, Mexico
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Prof Clara Gorodezky
Head of the Department of Immunology and Immunogenetics
Instituto de Diagnóstico y Referencia Epidemiológicos
InDRE, Secretary of Health
Carpio 470 1st floor
México D.F. 11340
Mexico
Tel: 52 55 5341 4569; 52 55 5342 7555
Fax: 52 55 5341 4418
e-mail: clarag@servidor.unam.mx

Abstract

Understanding the complex interaction between human leukocyte antigen (HLA) and killer immunoglobulin-like receptor (KIR) requires study of both HLA and KIR diversity in the same population. The presence of KIR genes 2DL1, 2, 3, 4, 5, KIR3DL1, 3DL2, 3DL3, KIR2DS1, 2DS2, 2DS3, 2DS4, 2DS5, KIR3DS1, KIR3DP1, KIR2DP1 was determined in 54 unrelated Mexican Mestizo donors. The PCR sequence-specific oligonucleotide probe One Lambda kit (Luminex) kindly given by J. Lee was used for typing. The software analyses the combination obtained for each of the five exons. Five controls (UCLA DNA exchange) were run as quality control. The gene frequency (GF) was calculated for the 16 KIR loci; the GF of individual genes was 100% for 2DL4, 3DL1, 3DL2, 3DL3, 3DP1. KIR2DL1 (76.43%), KIR2DL2 (37.64%), KIR2DL3 (76.43%), KIR2DL5 (29.29%), KIR3DS1 (23.02%), KIR2DS1 (21.83%), KIR2DS2 (37.64%), KIR2DS3 (50.93%), KIR2DS4 (86.93%), KIR2DS5 (29.29%), KIR2DP1 (86.39%). We observed similar frequencies with Caucasians and Mediterraneans, with exceptions: KIR3DL1 which was present in 100% Mexicans, ranged from 62% to 75% in Caucasians; 2DS3 (50.9%) vs 14–20% 2DS4 (86.39%) vs 65–79% and 2DS5 (29.29%) vs 11–18% in Caucasians. The finding of 23 phenotypes in 54 individuals accounting for both chromosomes, demonstrates the enormous diversity. We found 14 different combinations of stimulatory KIRs in the phenotypes; every subject had at least one stimulatory KIR; in all of them, 2DS4 existed except for one person who may have some new combination: 2DS2 2DS3. Extended family data will offer accurate and precise haplotypes to provide an insight on the significance of ethnic distribution and KIR repertoire.

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