PEPTIDE SEGMENT COUPLING IN AQUEOUS MEDIUM: SILVER ION ACTIVATION OF THE THIOLCARBOXYL GROUP

Authors


Hormone Research Laboratory 1088 HSW University of California San Francisco, CA 94143 U.S.A.

Abstract

The use of the thiolcarboxyl function for the assemblage of deblocked peptide segments in aqueous medium has been investigated. The C-terminal thiolcarboxyl peptide Ac-Tyr-Arg-Arg-Glu-Arg-Gly-SH (2a) has been synthesized by the solid-phase method. The silver compound of peptide 2a was coupled to H-Phe-Ala-Glu-Gly-OH in 50% aqueous dimethylformamide to give a 40% yield of Ac-Tyr-Arg-Arg-Glu-Arg-Gly-Phe-Ala-Glu-Gly-OH.

Ancillary