Simplified procedure for carrying out simultaneous multiple hydrogen fluoride cleavages of protected peptide resins


Department of Molecular Biology Scripps Clinic and Research Foundation 10666 N. Torrey Pines Road – MB8 La Jolla, CA 92037 USA


Methods have recently been presented which greatly increase the ability to synthesize large numbers of peptides. These advances make it essential to be able to cleave large numbers of protected peptide resins. Two different procedures are presented for carrying out cleavage of protected peptide resins. The first procedure enables multiple cleavages to be carried out with many existing HF apparatuses, while the second utilizes a new apparatus design. Using these procedures, at least 50 individual cleavages can be carried out per day.