Recombinant human brain-derived neurotrophic factor (rHuBDNF)

Disulfide structure and characterization of BDNF expressed in CHO cells


Address: Dr Mitsuru Haniu Amgen Center 1840 Dehavilland Drive Thousand Oaks, CA 91320-1789 USA


Three disulfide linkages of recombinant human brain-derived neurotrophic factor (BDNF) were determined by peptide sequence analysis and characterized by mass spectrometry. The three disulfide bonds for BDNF expressed in Chinese hamster ovary cells include Cys-13-Cys-80, Cys-58-Cys-109 and Cys-68-Cys-111, and the disulfide structure was homologous to that of nerve growth factor.