Interference with Aggregatibacter actinomycetemcomitans: colonization of epithelial cells under hydrodynamic conditions
Article first published online: 17 AUG 2009
© 2009 John Wiley & Sons A/S
Oral Microbiology and Immunology
Volume 24, Issue 5, pages 390–395, October 2009
How to Cite
Sliepen, I., Van Essche, M., Loozen, G., Van Eldere, J., Quirynen, M. and Teughels, W. (2009), Interference with Aggregatibacter actinomycetemcomitans: colonization of epithelial cells under hydrodynamic conditions. Oral Microbiology and Immunology, 24: 390–395. doi: 10.1111/j.1399-302X.2009.00531.x
- Issue published online: 17 AUG 2009
- Article first published online: 17 AUG 2009
- Accepted for publication April 27, 2009
- Aggregatibacter actinomycetemcomitans;
- bacterial interference;
- epithelial cells;
Introduction: Microbial interactions are considered to be important for bacterial colonization. Interactions that inhibit colonization of pathogens could possibly be used as a new treatment approach for periodontitis. The aim of this study was to test this hypothesis on soft surfaces in vitro, taking into account the hydrodynamic forces continuously present in vivo.
Methods: Cultured epithelial cells were precolonized with Streptococcus sanguinis KTH-4, Streptococcus cristatus CC5A, Streptococcus salivarius TOVE and Streptococcus mitis BMS before Aggregatibacter actinomycetemcomitans colonization. Experiments were performed in a modified Robbins-device-type flow cell. Bacterial colonization and the number of epithelial cells were evaluated by microbial culturing and quantitative polymerase chain reaction.
Results: The streptococci were able to inhibit A. actinomycetemcomitans colonization on soft tissue surfaces under flow conditions. Statistically significant differences were found between streptococcal pretreatments and the controls, with the most pronounced effect caused by S. sanguinis.
Conclusion: These data confirm the possibility of applying beneficial bacteria in periodontal treatment.