These authors contributed equally to this manuscript.
Association of early growth response-1 gene polymorphisms with total IgE and atopy in asthmatic children
Article first published online: 28 MAY 2008
© 2008 The Authors. Journal compilation © 2008 Blackwell Munksgaard
Pediatric Allergy and Immunology
Volume 20, Issue 2, pages 142–150, March 2009
How to Cite
Chan, I. H. S., Tang, N. L. S., Leung, T. F., Huang, W., Lam, Y. Y. O., Wong, G. W. K., Chan, J. C. N., Chan, M. H. M., Wong, C. K., Zhang, Y. P. and Lam, C. W. K. (2009), Association of early growth response-1 gene polymorphisms with total IgE and atopy in asthmatic children. Pediatric Allergy and Immunology, 20: 142–150. doi: 10.1111/j.1399-3038.2008.00757.x
- Issue published online: 25 MAR 2009
- Article first published online: 28 MAY 2008
- Accepted 2 May 2008
- early growth response-1;
- genetic polymorphism;
- immunoglobulin E
Early growth response-1 (Egr-1) is expressed in human airways and found to modulate tumor necrosis factor, immunoglobulin E (IgE), airway responsiveness, and interleukin-13-induced inflammation in mice. We investigated the effects of Chinese-tagging single nucleotide polymorphisms (SNPs) of Egr-1 on asthma traits in 298 Chinese asthmatic children and 175 controls, and a replication community cohort of 191 controls. Tag SNP (−4071 AG) and three additional SNPs (−1427 CT, −151 CT and IVS1 −42 CT) were genotyped by restriction fragment length polymorphism (RFLP). Significant associations were found between plasma total IgE concentration and −4071 AG (p = 0.008) and IVS1 −42 CT (p = 0.027) in asthmatic patients. After Bonferroni correction, only −4071 AG showed significant association. Multivariate regression analysis confirmed this significant association with a standardized coefficient β of 0.156 (95% CI: 0.046–0.317; p = 0.009) in asthmatics among the three SNPs with age and gender-adjusted. In −4071 AG, IgElog was significantly higher in patients with the GG genotype than the AA genotype (p = 0.009). In addition, −4071 AG was significantly associated with atopy (p = 0.016) and high total IgE concentration (p = 0.030) among asthmatics. Patients with the G allele had a 3.5-fold risk of having atopy and a 2.0-fold risk of having high total IgE concentration than those homozygous for the A allele. This is the first report to show significant association of Egr-1 polymorphisms with plasma total IgE and atopy in asthmatics. It may help to explore the pharmacogenetics of Egr-1 inhibitors.