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Increased serum interleukin-17 and peripheral Th17 cells in children with acute Henoch–Schönlein purpura

Authors

  • Hsiao-Yu Jen,

    1. Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, Taipei, Taiwan
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    • These two authors contributed equally to the manuscript.

  • Ya-Hui Chuang,

    1. Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, Taipei, Taiwan
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    • These two authors contributed equally to the manuscript.

  • Sheng-Chieh Lin,

    1. Department of Pediatrics, Shuang Ho Hospital, Taipei Medical University, Taipei, Taiwan
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  • Bor-Luen Chiang,

    1. Department of Pediatrics, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan
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  • Yao-Hsu Yang

    1. Department of Pediatrics, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan
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Yao-Hsu Yang, Department of Pediatrics, National Taiwan University Hospital, No. 7 Chung-Shan South Road, Taipei, Taiwan.
Tel.: 886 2 2312 3456 ext. 71717
Fax: 886 2 2311 9087
E-mail: yan0126@ms15.hinet.net

Abstract

To cite this article: Jen H-Y, Chuang Y-H, Lin S-C, Chiang B-L, Yang Y-H. Increased serum interleukin-17 and peripheral Th17 cells in children with acute Henoch–Schönlein purpura. Pediatr Allergy Immunol 2011: 22: 862–868.

Abstract

Background:  Interleukin (IL)-17 and Th17 cells have been involved in many autoimmune diseases. The aim of this study is to investigate the involvement of IL-17 and Th17 cells in the pathogenesis of childhood Henoch–Schönlein purpura (HSP).

Methods:  Serum and supernatant levels of cytokines and chemokines were analyzed by enzyme-linked immunosorbent assay (ELISA). Using intracellular staining, the frequency of peripheral Th17 and Th1 cells was studied by flow cytometry.

Results:  Children with acute HSP had significantly higher serum levels of IL-17, IL-6 and transforming growth factor-β than healthy controls. The IL-17 levels in culture supernatants of peripheral blood mononuclear cells with anti-CD3 and CD28 antibody stimulation were much higher in patients with HSP (281.2 ± 91.4 vs. 47.7 ± 22.6 pg/ml, p = 0.022). The patients also had more Th17 cells (1.67 ± 0.36% vs. 0.71 ± 0.15%, p = 0.033) but not Th1 cells in peripheral blood. Moreover, IL-17 could promote human endothelial cells to produce chemoattractants IL-8 and monocyte chemotactic protein-1.

Conclusion:  The increased frequency of peripheral Th17 cells and serum IL-17 levels are shown in childhood HSP that may in part contribute to vascular inflammation, suggesting cellular immunity is likely to be involved in the process of HSP.

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