Decrease in interleukin-21 in children suffering with severe atopic dermatitis

Authors

  • Sheng-Chieh Lin,

    1. Department of Pediatrics, Shuang Ho Hospital, Taipei Medical University, Taipei, Taiwan
    2. Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan
    Search for more papers by this author
  • Ya-Hui Chuang,

    1. Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, Taipei, Taiwan
    Search for more papers by this author
  • Yao-Hsu Yang,

    1. Department of Pediatrics, National Taiwan University Hospital, Taipei, Taiwan
    Search for more papers by this author
  • Bor-Luen Chiang

    1. Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan
    2. Department of Pediatrics, National Taiwan University Hospital, Taipei, Taiwan
    Search for more papers by this author

Dr. Bor-Luen Chiang, Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, No.7.Chung San South Road, Taipei, Taiwan.
Tel.: 886 2 23123456 ext 67302
Fax: 886 2 23119087
E-mail: gicmbor@ntu.edu.tw

Abstract

To cite this article: Lin S-C, Chuang Y-H, Yang Y-H, Chiang B-L. Decrease in interleukin-21 in children suffering with severe atopic dermatitis. Pediatr Allergy Immunol 2011: 22: 869–875.

Abstract

Background:  Atopic dermatitis (AD) is a kind of eczema with an inflammatory, relapsing, non-contagious, and pruritic skin disorder. It is associated with the local infiltration of T helper type 2 (Th2) cells that secrete interleukin (IL)-4 and IL-5. IL-21 is a member of IL-2 family cytokine mainly expressed by activated CD4+ T lymphocytes. Until now, there is no clinical research in the expression of IL-21 in patients with AD.

Methods:  We analyzed serum levels of total immunoglobulin E (IgE), allergen-specific IgE, and cytokines IL-4, IL-5, IFN-γ, IL-17, and IL-21 in AD cases and controls. In addition, cytokine levels in the culture supernatants of peripheral blood mononuclear cells stimulated with anti-CD3 and anti-CD28 Abs, phytohemagglutin (PHA), or pokeweed mitogen (PWM) were measured. We also assessed clinical skin severity by Scoring of Atopic Dermatitis (SCORAD) index.

Results:  Our results showed that serum total IgE in the case group was significantly higher than that of control group (365.449 ± 52.945 and 39.243 ± 7.605 IU/ml, respectively). Logistic regression analysis system reveals serum levels of IL-21 and IFN-γ are significantly correlated. However, IL-21 and IL-4, IL-21 and IL-5, as well as IL-21 and IL-17 showed no correlation.

Conclusion:  A significantly decreased level of IL-21 was observed in children suffering with severe AD compared with controls, suggesting that IL-21 may play a role in AD.

Ancillary