These authors contributed equally to this work.
Responses of CD4+CD25+Foxp3+ and IL-10-secreting type I T regulatory cells to cluster-specific immunotherapy for allergic rhinitis in children
Article first published online: 23 DEC 2011
© 2011 John Wiley & Sons A/S
Pediatric Allergy and Immunology
Volume 23, Issue 2, pages 141–150, March 2012
How to Cite
Lou, W., Wang, C., Wang, Y., Han, D. and Zhang, L. (2012), Responses of CD4+CD25+Foxp3+ and IL-10-secreting type I T regulatory cells to cluster-specific immunotherapy for allergic rhinitis in children. Pediatric Allergy and Immunology, 23: 141–150. doi: 10.1111/j.1399-3038.2011.01249.x
- Issue published online: 21 MAR 2012
- Article first published online: 23 DEC 2011
- Accepted for publication 29 October 2011
- allergic rhinitis;
- peripheral tolerance;
- regulatory T cells;
- specific immunotherapy;
- Tr1 cells
To cite this article: Lou W, Wang C, Wang Y, Han D, Zhang L. Responses of CD4+CD25+Foxp3+ and IL-10-secreting type I T regulatory cells to cluster-specific immunotherapy for allergic rhinitis in children. Pediatric Allergy Immunology 2011: doi: 10.1111/j.1399-3038.2011.01249.x.
We investigated the effects of cluster specific immunotherapy (SIT) with Dermatophagoides pteronyssinus (Der p) on CD4+CD25+Foxp3+ Treg cells and IL-10-secreting type I T regulatory (Tr1) cells in Der p-sensitized children with allergic rhinitis (AR). We performed a prospective randomized study involving 46 children (aged 8–13 yr), of whom 25 children received Der p-SIT + pharmacotherapy and 21 received only pharmacotherapy, over a period of 1 yr. Prior to and at end of treatment, CD4+CD25+Foxp3+ Treg cells and allergen-specific IL-10+IL-4−, IFN-γ+IL-4−, and IL-4+IFN-γ-CD4+ T cells were measured by flow cytometry. Similarly, IL-4, IFN-γ, and IL-10 in supernatants from allergen-stimulated peripheral blood mononuclear cell (PBMC) cultures were measured by ELISA, and the suppressive effect of CD4+CD25high T cells on cell proliferation and cytokine release was estimated from both groups. Allergen-specific serum IgE and IgG4 were also assessed at the beginning and end of treatment by RAST and ELISA, respectively. The levels of allergen-specific Tr1 cells, IgG4, and allergen-induced IL-10 synthesis from PBMC cultures were significantly increased after SIT for 1 yr compared with baseline levels (p < 0.001 for all), with significant correlation between increased levels of Tr1 cells and improvements in nasal symptoms (r = 0.48, p < 0.05). In contrast, the levels of CD4+CD25+Foxp3+ T cells, allergen-specific Th1 and Th2 cells, the production of IL-4 and IFN-γ, and the function of CD4+CD25high T cells were not altered in either group at the end of treatment. These data suggest that the up-regulation of Tr1 cells may play an important role in SIT and be a useful marker of successful SIT in AR patients.