Dark-Germination of Reciprocal Hybrid Seed from Light-Requiring and -Indifferent Nicotiana tabacum


  • M. J. Kasperbauer

    1. Crops Research Division, Agricultural Research Service, U.S. Department of Agriculture; and Kentucky Agricultural Experiment Station, Lexington, Kentucky 40506
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Seed resulting from self-fertilization of Nicotiana tabacum plants was tested for germination in uninterrupted darkness at monthly intervals beginning at time of harvest. All seed lots were light-requiring immediately after harvest. Some lots continued to be light-requiring, but others gradually became light-indifferent during storage under laboratory conditions. One light-requiring and one light-indifferent selection were self-pollinated, and reciprocally crossed. The seed resulting from self-fertilization was light-requiring or light-indifferent, respectively, according to the parentage. Seed resulting from the reciprocal crosses differed in dark-germinability. Further, dark-germination of seed from each of the reciprocal crosses differed from that of either parent. Both parents contributed toward light sensitivity of the seed; however, contribution of the maternal parent was greater than that of the pollen parent.