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Abstract

Embryo formation was induced in anther cultures of Anemone virginiana and some other species of the same genus. Activated charcoal was included in the culture medium which gave the higher percentage embryos. When the cytokinin 2ip was included (10−6M) embryo formation was also induced but at a lower percentage. This indicates that abscisic acid (ABA) can be involved in the normal inhibition of embryo formation in pollen.