Plant Regeneration from Mesophyll Protoplasts of Several Nicotiana Species

Authors

  • JEAN-PIERRE BOURGIN,

    1. Laboratoire de Biologie cellulaire, Département de Physiologie végétale, Institut National de la Recherche Agronomique, F 78000 Versailles, France
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  • YVES CHUPEAU,

    1. Laboratoire de Biologie cellulaire, Département de Physiologie végétale, Institut National de la Recherche Agronomique, F 78000 Versailles, France
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  • CLAUDINE MISSONIER

    1. Laboratoire de Biologie cellulaire, Département de Physiologie végétale, Institut National de la Recherche Agronomique, F 78000 Versailles, France
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Abstract

In a search for model systems in plant cell genetics studies mesophyll protoplasts from eleven species of Nicotiana with low chromosome number (N. acuminata, N. alata, N. glauca, N. glutinosa, N. langsdorffii, N. longiflora, N. otophora, N. paniculata, N. plumbaginifolia, N. suaveolens, N. sylvestris) were shown to divide in a liquid culture medium. Plants were recovered from calli originating from protoplasts of all these species except N. glutinosa.

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