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Identification and quantification of endogenous gibberellins in apical buds and the cambial region of Eucalyptus

Authors

  • O. Hasan,

    1. O. Hasan and J.B. Reid (corresponding author). Cooperative Research Centre for Temperate Hardwood Forestry and Dept of Plant Science. The Univ. of Tasmania. Hobart. Tasmania 7001. Australia: B.G. Ridoutt, Cooperative Research Centre for Hardwood Fibre and Paper Science. School of Forestry, The Univ of Melbourne, Crewick. Victoria 3363. Australia; J.J. Ross, Dept of Plant Science. N. W. Davies. Central Science Laboratory. The Univ. of Tasmania. Hobart. Tasman a 7001. Australia.
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  • B.G. Ridoutt,

    1. O. Hasan and J.B. Reid (corresponding author). Cooperative Research Centre for Temperate Hardwood Forestry and Dept of Plant Science. The Univ. of Tasmania. Hobart. Tasmania 7001. Australia: B.G. Ridoutt, Cooperative Research Centre for Hardwood Fibre and Paper Science. School of Forestry, The Univ of Melbourne, Crewick. Victoria 3363. Australia; J.J. Ross, Dept of Plant Science. N. W. Davies. Central Science Laboratory. The Univ. of Tasmania. Hobart. Tasman a 7001. Australia.
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  • J.J. Ross,

    1. O. Hasan and J.B. Reid (corresponding author). Cooperative Research Centre for Temperate Hardwood Forestry and Dept of Plant Science. The Univ. of Tasmania. Hobart. Tasmania 7001. Australia: B.G. Ridoutt, Cooperative Research Centre for Hardwood Fibre and Paper Science. School of Forestry, The Univ of Melbourne, Crewick. Victoria 3363. Australia; J.J. Ross, Dept of Plant Science. N. W. Davies. Central Science Laboratory. The Univ. of Tasmania. Hobart. Tasman a 7001. Australia.
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  • N.W. Davies,

    1. O. Hasan and J.B. Reid (corresponding author). Cooperative Research Centre for Temperate Hardwood Forestry and Dept of Plant Science. The Univ. of Tasmania. Hobart. Tasmania 7001. Australia: B.G. Ridoutt, Cooperative Research Centre for Hardwood Fibre and Paper Science. School of Forestry, The Univ of Melbourne, Crewick. Victoria 3363. Australia; J.J. Ross, Dept of Plant Science. N. W. Davies. Central Science Laboratory. The Univ. of Tasmania. Hobart. Tasman a 7001. Australia.
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  • J.B. Reid

    1. O. Hasan and J.B. Reid (corresponding author). Cooperative Research Centre for Temperate Hardwood Forestry and Dept of Plant Science. The Univ. of Tasmania. Hobart. Tasmania 7001. Australia: B.G. Ridoutt, Cooperative Research Centre for Hardwood Fibre and Paper Science. School of Forestry, The Univ of Melbourne, Crewick. Victoria 3363. Australia; J.J. Ross, Dept of Plant Science. N. W. Davies. Central Science Laboratory. The Univ. of Tasmania. Hobart. Tasman a 7001. Australia.
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Abstract

Endogenous gibberellins (GAs) were extracted and purified from apical buds of Eucalyptus nitens (Deane and Maid.) Maid. and the cambial region of E. globulus (Labill.). then analysed by capillary gas chromatography-mass spectrometry. GA1 GA19 GA20 and GA29 were identified by full scan mass spectra. Kovats retention indices and high resolution selected ion monitoring. Using deuterated internal standards. GA1. GA19. GA20 and putative GA29 and GA53 were quantified in the apical buds, while GA4. GA8. GA9 and GA44 were shown to be either absent or present at very low levels. From the cambial region. GA1 and GA20 were quantified at levels of 0.30 ng (g fresh weight)-1 and 8.8 ng (g fresh weight)-1 respectively. These data suggest that the early 13-hydroxylation pathway is the dominant pathway for GA biosynthesis in Eucalyptus.

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