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Keywords:

  • Carrot;
  • cell wall proteins;
  • Daucus carota;
  • extensin;
  • hydroxyproline-rich;
  • immunolocalization;
  • Lycopersicon esculentum;
  • potato;
  • potato tuber lectin;
  • Solanum tuberosum;
  • tomato

Tissue-specific expression of two members of the cell wall hydroxyproline-rich glycoprotein (HRGP) family, extensin and potato tuber lectin, was examined by immunolocalization at the light microscope level in various organs (leaves, stems, roots, fruit, tuber) of carrot (Daucus carota cv. Thumbelina), tomato (Lycopersicon esclentum cv. Pixie Hybrid II), and potato (Solanum tuberosum cv. Kennebec). Extensin was prominently expressed in vascular tissue, particularly xylem and also phloem, although virtually all cells displayed some degree of staining which varied as a function of the tissue, organ, and plant under study. Antibodies against potato tuber lectin (PTL) displayed a localization pattern similar to that observed for extensin; notably PTL did not stain cambium but did stain epithelial cells lining secretory cavities. These distribution patterns are consistent with a role for extensin, and possibly PTL, in providing mechanical support in tissues subjected to compression or torsional stress imparted by vascular growth, or by similar stress brought about by transport of vascular fluids.