Comparative proteome analysis of differentially expressed proteins induced by Al toxicity in soybean

Authors

  • Yan Zhen,

    1. Institute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China
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    • These authors contributed equally to this work.

  • Jin-Liang Qi,

    1. Institute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China
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    • These authors contributed equally to this work.

  • Si-Si Wang,

    1. Institute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China
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  • Jing Su,

    1. Institute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China
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  • Guo-Hua Xu,

    1. Institute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China
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  • Ming-Sheng Zhang,

    1. Institute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China
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  • Lv Miao,

    1. Institute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China
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  • Xin-Xiang Peng,

    1. College of Life Sciences, South China Agricultural University, Guangzhou 516042, China
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  • Dacheng Tian,

    1. Institute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China
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  • Yong-Hua Yang

    Corresponding author
    1. Institute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China
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    • Present address: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China


*e-mail: yangyh@nju.edu.cn

Abstract

Phytotoxic aluminum (Al) is a limiting factor for crop production on acid soils. The molecular mechanism, however, underlying Al toxicity and responses in plants is still not well understood. We report here the characterization of comparative proteome of aluminum-stress-responsive proteins in a known Al-resistant soybean cultivar, Baxi 10 (BX10). To investigate time-dependent responses, 1-week-old soybean seedlings were exposed to 50 μM AlCl3 for 24, 48 and 72 h, and total proteins extracted from roots were separated by two-dimensional electrophoresis. More than 1200 root proteins of the soybean BX10 seedling were reproducibly resolved on the gels. A total of 39 differentially expressed spots in abundance were identified by mass spectrometry, with 21 upregulated, 13 newly induced and 5 downregulated. The heat shock protein, glutathione S-transferase, chalcone-related synthetase, GTP-binding protein and ABC transporter ATP-binding protein were previously detected at the transcriptional or translational level in other plants. Other proteins, identified in this study, are new Al-induced proteins. Soybean BX10 roots under aluminum stress could be characterized by the cellular activities involved in stress/defense, signal transduction, transport, protein folding, gene regulation, and primary metabolisms, which are critical for plant survival under Al toxicity. This present study expands our understanding of differentially expressed proteins associated with aluminum stress on soybean BX10.

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