These authors have contributed equally to this work.
How reliable is the double-ended pressure sleeve technique for assessing xylem vulnerability to cavitation in woody angiosperms?
Article first published online: 13 APR 2011
Copyright © Physiologia Plantarum 2011
Volume 142, Issue 3, pages 205–210, July 2011
How to Cite
Ennajeh, M., Simões, F., Khemira, H. and Cochard, H. (2011), How reliable is the double-ended pressure sleeve technique for assessing xylem vulnerability to cavitation in woody angiosperms?. Physiologia Plantarum, 142: 205–210. doi: 10.1111/j.1399-3054.2011.01470.x
- Issue published online: 12 JUN 2011
- Article first published online: 13 APR 2011
- Accepted manuscript online: 14 MAR 2011 12:55PM EST
- Received 27 October 2010; revised 17 January 2011
The reliability of a double-ended pressure sleeve technique was evaluated on three woody angiosperm species with contrasting maximum vessel lengths. Vulnerability curves (VCs) were constructed by varying sample length and the size of the pressure sleeves. VCs were compared against curves obtained with reference techniques. For the two diffuse-porous species, Betula pendula and Prunus persica, VCs built with shoot segments shorter than maximum vessel length strongly overestimated species vulnerability. Furthermore, increasing the size of the pressure sleeve also tended to lead to overestimated VCs. For the ring-porous species Quercus robur, the technique strongly overestimated vulnerability to embolism, whatever the sample length or chamber tested. In conclusion, the double-ended pressure sleeve technique only gives reliable VCs on diffuse-porous angiosperms with short pressure sleeves, only when segments are longer than maximum vessel length.