Antioxidant enzyme activities and hormonal status in response to Cd stress in the wetland halophyte Kosteletzkya virginica under saline conditions

Authors

  • Rui-Ming Han,

    1. Groupe de Recherche en Physiologie végétale (GRPV), Earth and Life Institute – Agronomy (ELI-A), Université catholique de Louvain (UCL), Louvain-la-Neuve, Belgium
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  • Isabelle Lefèvre,

    1. Groupe de Recherche en Physiologie végétale (GRPV), Earth and Life Institute – Agronomy (ELI-A), Université catholique de Louvain (UCL), Louvain-la-Neuve, Belgium
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  • Alfonso Albacete,

    1. Departamento de Nutrición Vegetal, Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), Campus Universitario de Espinardo, Murcia, Spain
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  • Francisco Pérez-Alfocea,

    1. Departamento de Nutrición Vegetal, Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), Campus Universitario de Espinardo, Murcia, Spain
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  • Gregorio Barba-Espín,

    1. Grupo de Biotecnología de Frutales, Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), Campus Universitario de Espinardo, E-30100, Murcia, Spain
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  • Pedro Díaz-Vivancos,

    1. Grupo de Biotecnología de Frutales, Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), Campus Universitario de Espinardo, E-30100, Murcia, Spain
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  • Muriel Quinet,

    1. Groupe de Recherche en Physiologie végétale (GRPV), Earth and Life Institute – Agronomy (ELI-A), Université catholique de Louvain (UCL), Louvain-la-Neuve, Belgium
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  • Cheng-Jiang Ruan,

    1. Key laboratory of Biotechnology and Bio-resources Utilisation, State Ethnic Affairs Commission and Ministry of Education, Dalian Nationalities University, Dalian, China
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  • José Antonio Hernández,

    1. Grupo de Biotecnología de Frutales, Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), Campus Universitario de Espinardo, E-30100, Murcia, Spain
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  • Elena Cantero-Navarro,

    1. Departamento de Nutrición Vegetal, Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), Campus Universitario de Espinardo, Murcia, Spain
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  • Stanley Lutts

    Corresponding author
    • Groupe de Recherche en Physiologie végétale (GRPV), Earth and Life Institute – Agronomy (ELI-A), Université catholique de Louvain (UCL), Louvain-la-Neuve, Belgium
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Correspondence

Corresponding author

e-mail: stanley.lutts@uclouvain.be

Abstract

Salt marshes constitute major sinks for heavy metal accumulation but the precise impact of salinity on heavy metal toxicity for halophyte plant species remains largely unknown. Young seedlings of Kosteletzkya virginica were exposed during 3 weeks in nutrient solution to Cd 5 µM in the presence or absence of 50 mM NaCl. Cadmium (Cd) reduced growth and shoot water content and had major detrimental effect on maximum quantum efficiency (Fv/Fm), effective quantum yield of photosystem II (Y(II)) and electron transport rates (ETRs). Cd induced an oxidative stress in relation to an increase in O2•− and H2O2 concentration and lead to a decrease in endogenous glutathione (GSH) and α-tocopherol in the leaves. Cd not only increased leaf zeatin and zeatin riboside concentration but also increased the senescing compounds 1-aminocyclopropane-1-carboxylic acid (ACC) and abscisic acid (ABA). Salinity reduced Cd accumulation already after 1 week of stress but was unable to restore shoot growth and thus did not induce any dilution effect. Salinity delayed the Cd-induced leaf senescence: NaCl reduced the deleterious impact of Cd on photosynthesis apparatus through an improvement of Fv/Fm, Y(II) and ETR. Salt reduced oxidative stress in Cd-treated plants through an increase in GSH, α-tocopherol and ascorbic acid synthesis and an increase in glutathione reductase (EC 1.6.4.2) activity. Additional salt reduced ACC and ABA accumulation in Cd+NaCl-treated leaves comparing to Cd alone. It is concluded that salinity affords efficient protection against Cd to the halophyte species K. virginica, in relation to an improved management of oxidative stress and hormonal status.

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