These authors contributed equally to this work.
Trehalose determination in linseed subjected to osmotic stress. HPAEC-PAD analysis: an inappropriate method
Article first published online: 13 SEP 2012
Copyright © Physiologia Plantarum 2012
Volume 147, Issue 3, pages 261–269, March 2013
How to Cite
Quéro, A., Béthencourt, L., Pilard, S., Fournet, A., Guillot, X., Sangwan, R. S., Boitel-Conti, M., Courtois, J. and Petit, E. (2013), Trehalose determination in linseed subjected to osmotic stress. HPAEC-PAD analysis: an inappropriate method. Physiologia Plantarum, 147: 261–269. doi: 10.1111/j.1399-3054.2012.01677.x
- Issue published online: 14 FEB 2013
- Article first published online: 13 SEP 2012
- Accepted manuscript online: 17 AUG 2012 10:38AM EST
- Manuscript Accepted: 12 JUN 2012
- Manuscript Revised: 7 MAY 2012
- Manuscript Received: 7 FEB 2012
- La Région Picardie, France
Trehalose is a non-reducing disaccharide involved in stress tolerance in plants. To understand better the role of trehalose in the osmotic stress response in linseed (Linum usitatissimum), trehalose content in leaves was studied. First, the method commonly used for sugar determination, high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), gave unsatisfactory results and the separation efficiency could not be improved by varying the elution conditions. The same problem was also found in the model plant: Arabidopsis thaliana. After clearly highlighting a co-elution of trehalose in these two species by a trehalase assay and liquid chromatography-high resolution mass spectrometry analysis, gas chromatography–mass spectrometry (GC-MS) was used as the analytical method instead. These results confirmed that trehalose content is currently overestimated by HPAEC-PAD analysis, approximately 7 and 13 times for A. thaliana and linseed respectively. Thus GC-MS gave more satisfactory results for trehalose quantification in plants. With this method, trehalose accumulation was observed in linseed during an osmotic stress (−0.30 MPa), the quantity (31.49 nmol g–1 dry weight after 48 h) appears too low to assign an osmoprotector or osmoregulator role to trehalose in stressed linseed.