These authors contributed equally to this article.
Roles of cell walls and intracellular contents in supercooling capability of xylem parenchyma cells of boreal trees
Version of Record online: 13 SEP 2012
Copyright © Physiologia Plantarum 2012
Volume 148, Issue 1, pages 25–35, May 2013
How to Cite
Kasuga, J., Endoh, K., Yoshiba, M., Taido, I., Arakawa, K., Uemura, M. and Fujikawa, S. (2013), Roles of cell walls and intracellular contents in supercooling capability of xylem parenchyma cells of boreal trees. Physiologia Plantarum, 148: 25–35. doi: 10.1111/j.1399-3054.2012.01678.x
- Issue online: 15 APR 2013
- Version of Record online: 13 SEP 2012
- Accepted manuscript online: 17 AUG 2012 10:40AM EST
- Manuscript Accepted: 12 JUN 2012
- Manuscript Revised: 23 MAY 2012
- Manuscript Received: 26 MAR 2012
The supercooling capability of xylem parenchyma cells (XPCs) in boreal hardwood species differs depending not only on species, but also season. In this study, the roles of cell walls and intracellular contents in supercooling capability of XPCs were examined in three boreal hardwood species, Japanese beech, katsura tree and mulberry, whose supercooling capability differs largely depending on species and season. XPCs in these species harvested in winter and summer were treated by rapid freezing and thawing (RFT samples) or by RFT with further washing (RFTW samples) to remove intracellular contents from XPCs in order to examine the roles of cell walls in supercooling. RFT samples were also treated with glucose solution (RFTG samples) to examine roles of intracellular contents in supercooling. The supercooling capabilities of these samples were examined by differential thermal analysis after ultrastructural observation of XPCs by a cryo-scanning electron microscope to confirm effects of the above treatments. XPCs in RFTW samples showed a large reduction in supercooling capability to similar temperatures regardless of species or season. On the other hand, XPCs in RFTG samples showed a large increase in supercooling capability to similar temperatures regardless of species or season. These results indicate that although cell walls have an important role in maintenance of supercooling, change in supercooling capability of XPCs is induced by change in intracellular contents, but not by change in cell wall properties.