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ppl1704-sup-0001-TableS1.docWord document51K Table S1. Primers for homozygous mutants screening and RT-PCR analysis.
ppl1704-sup-0002-FigureS1.tifTIFF image5695K Fig. S1. Identification and characterization of the homozygous mag2 and mag2l knockout mutants. Homozygous T-DNA insertion mutants were confirmed by PCR screening wiht gene specific primers. Homozygous mag2 and mag2l mutants are knockout mutants as confirmed by RT-PCR analysis.
ppl1704-sup-0003-FigureS2.tifTIFF image3170K Fig. S2. Germination rate of wt, mag2 and mag2l seeds with different treatments. Germination rates of wt, mag2, and mag2l seeds in the presence of various concentrations of NaCl, KCl, Mannitol, ABA, and LiCl at 7 days after stratification were shown. Root growth of wt, mag2, and mag2l seedling with ABA, NaCl, and Mannitol treatments was shown. Photographs were taken at the end of treatments.
ppl1704-sup-0004-FigureS3.tifTIFF image7383K Fig. S3. Complementary assays. The phenotypes of mag2 during germination and seedling establishment under various treatments were complemented by CDS of MAG2 and were partially complemented by CDS of MAG2L.
ppl1704-sup-0005-FigureS4.tifTIFF image2183K Fig. S4. Physical interaction between MAG2 and ZW10CR. Y2H assay was used to determine the interaction among MAG2, MAG2L, ZW10, and three fragments of ZW10 protein.
ppl1704-sup-0006-FigureS5.tifTIFF image767K Fig. S5. BiP2::GUS expression patterns in wt, mag2, mag2l and zw10 seedlings with or without DTT treatment.
ppl1704-sup-0007-FigureS6.tifTIFF image268K Fig. S6. Root growth of wt, mag2 and mag2l seedlings with various concentrations K+ treatments.
ppl1704-sup-0008-FigureS7.tifTIFF image811K Fig. S7. Phylogeny of MAG2 and MAG2L.

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