Effect of long-term culture on the expression of antigens and adhesion molecule in single porcine pancreatic endocrine cells

  1. Kazuya Edamura1,2,
  2. Koko Nasu2,
  3. Yukiko Iwami2,
  4. Ryohei Nishimura3,
  5. Hiroyuki Ogawa1,
  6. Nobuo Sasaki3,
  7. Hisako Ohgawara2

Article first published online: 7 JUN 2005

DOI: 10.1111/j.1399-3089.2005.00232.x

Issue

Xenotransplantation

Xenotransplantation

Volume 12, Issue 4, pages 327–332, July 2005

Additional Information

How to Cite

Edamura, K., Nasu, K., Iwami, Y., Nishimura, R., Ogawa, H., Sasaki, N. and Ohgawara, H. (2005), Effect of long-term culture on the expression of antigens and adhesion molecule in single porcine pancreatic endocrine cells. Xenotransplantation, 12: 327–332. doi: 10.1111/j.1399-3089.2005.00232.x

Author Information

  1. 1

    Laboratory of Veterinary Emergency Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo

  2. 2

    Division of Cell Replacement and Regenerative Medicine, Medical Research Institute, School of Medicine, Tokyo Women's Medical University

  3. 3

    Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan

*Address reprint requests to Kazuya Edamura, Laboratory of Veterinary Surgery, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa city, Kanagawa Pref., 252-8510, Japan (E-mail: edamura@brs.nihon-u.ac.jp)

Publication History

  1. Issue published online: 7 JUN 2005
  2. Article first published online: 7 JUN 2005
  3. Received 15 June 2004; Accepted 10 March 2005

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Keywords:

  • culture;
  • Galα1-3Gal;
  • ICAM-1;
  • porcine pancreatic endocrine cell;
  • SLA class II

Abstract:  Background:  Changes in the expression of galactose (Gal) α1-3Gal, swine lymphocyte antigen (SLA) class II and intracellular adhesion molecule (ICAM)-1 of single porcine pancreatic endocrine (PE) cells during the culture period were investigated.

Methods:  Cultured porcine PE-cells were fixed in 10% buffered formalin for histological evaluation. At 1, 3, 6, 9 and 12 weeks of culture, mRNA was obtained from porcine PE-cells so that the expression of SLA class II and ICAM-1 genes could be examined by reverse transcriptase-polymerase chain reaction.

Results:  The rates of Galα1-3Gal and SLA class II-positive cells did not decrease during the culture period, but the rates of Galα1-3Gal and SLA class II strongly positive cells significantly decreased. ICAM-1-positive cells were scarcely observed during the culture period. SLA class II and ICAM-1 mRNAs were detected at 1 and 3 weeks of culture, but were not detected after 6 weeks of culture.

Conclusions:  These results suggest that partial reduction in the expression of these antigens could be obtained by a long-term culture.

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