Human MIP synthase splice variants in bipolar disorder


  • The authors of this paper do not have any commercial associations that might pose a conflict of interest in connection with this manuscript.

Galila Agam, PhD, Psychiatry Research Unit, Mental Health Center, PO Box 4600, Beer Sheva 84170, Israel. Fax: +972 7 640 1737; e-mail:


Objectives:  Alternative splicing allows the production of multiple gene products with different functions from a given sequence, affecting cellular function control. Tissue-specific splicing is most prevalent in the brain. We therefore investigate whether splice variants contribute to complex psychiatric disorders. A database search suggested that the myo-inositol-1-phosphate (MIP) synthase gene, possibly involved in pathophysiology of bipolar disorder, has splice variants.

Methods:  Human RNA was purified from lymphocytes and postmortem brain. MIP synthase alternative splice variants were amplified using reverse transcription-polymerase chain reaction.

Results:  The bioinformatics finding was confirmed in both tissues. No difference in lymphocyte MIP synthase mRNA splice-variant levels was found between bipolar patients and controls. However, patients with family history of a major psychiatric disorder had significantly higher levels of the variant lacking exons 3 and 4 versus patients with no family history and controls.

Conclusions:  As alternative splicing may be a mechanism by which the ∼30,000 genes are amplified in mammalian brain, further studies with other candidate genes for psychiatric disorders are needed.