Table S1 Mean July nest temperature was used in the analyses presented in the manuscript, but alternative measures of temperature were also consistent with our measures of effective heritability.

Table S2 Results from an ordinal logistic general linear model run to assess the significance of box of incubation, family, treatment, and treatment-by-family interaction on hatchling sex of the turtle Chrysemys picta.

Table S3 Parameters used to calculate heritability of the sex determination threshold via equations in Bull et al. (1982; p. 335).

Appendix S1 R code used to run MCMCglmm.

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