Table S1 Description of the Holocnemus pluchei samples: location, sample size and prevalence of Cardinium.

Table S2 Genes and primer features.

Figure S1 Numbers of eggs regressed against mother’s tibia-patella length.

Figure S2 Variation of Cardinium frequencies in the south of France and Spain.

Figure S3 Cardinium phylogeny constructed via maximum-likelihood using (a) 16S rRNA and (b) gyrb sequences.

Figure S4 Distribution of mtDNA haplotypes among populations, partitioned by Cardinium infection status.

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