Table S1 Primer sequences for the PCR amplification of MHC class I and class II DRB loci in the Alpine marmot (Marmota marmota).

Table S2 The PCR conditions and polymorphism characterization of MHC class I and class II DRB loci in Marmota marmota.

Table S3 The allele frequencies of four polymorphic loci in Marmota marmota.

Table S4 Observed (Ho), expected (He) heterozygosities and allelic richness (R) per putative locus per population.

Table S5 The amino acid sequences of MHC class I 2nd exon alleles (a) and of MHC class II DRB 2nd exon alleles (b) in Marmota marmota.

Table S6 The average pairwise nucleotide distances (Kimura 2-parameter models, K2P), Poisson-corrected amino acid distances, the average rates of synonymous (dS) and nonsynonymous (dN) substitutions per site and the results of Z-test of positive selection for polymorphic putative MHC loci in the Alpine marmot.

Table S7 The evaluation of the fit of various models of codon evolution.

Table S8 Marmota marmota MHC class I sequences.

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