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jeb2564-sup-0001-TableS1-S5-FigS2-S2.docWord document702KTable S1 Site locations and climatic data$ for populations of Drosophila simulans used clinal analysis of thermo-tolerance. Table S2 Site locations and populations statistics for Drosophila simulans analysed with microsatellite markers. Table S3 Principal Components analysis on temperature and variability data (see Table S1 for codes). Percentages for each PC shown in parenthesis. Table S4 Pairwise FST (above diagonal) and DEST (below diagonal) values. Table S5 Estimates of gene flow (θ) between populations (latitude below in brackets) of Drosophila simulans. The range estimates given below each value are the 95% confidence limits. Figure S1 Comparison of ramping temperature increase at a ramping rate of 0.06 °C min−1(open squares) with actual increases in mean shaded temperature between the 22nd and 24th of January 2011 in Melbourne south-eastern Victoria Australia (solid circles), (www.bom.gov.au). Figure S2 Summary plot of the proportional membership for each individual in each of four genetic clusters (K) for K = 4.

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