Erythrocyte Membrane Vesiculation and Changes in Membrane Composition during Storage in Citrate-Phosphate-Dextrose-Adenine-1

Authors

  • Tibor J. Greenwalt,

    Corresponding author
    1. Hoxworth Blood Center and Department of Medicine, University of Cincinnati, Ohio, USA
      Hoxworth Blood Center and Department of Medicine, University of Cincinnati, 3231 Burnet Avenue, Cincinnati, OH 45267 (USA)
    Search for more papers by this author
  • Donna J. Bryan,

    1. Hoxworth Blood Center and Department of Medicine, University of Cincinnati, Ohio, USA
    Search for more papers by this author
  • Umakant J. Dumaswala

    1. Hoxworth Blood Center and Department of Medicine, University of Cincinnati, Ohio, USA
    Search for more papers by this author

Hoxworth Blood Center and Department of Medicine, University of Cincinnati, 3231 Burnet Avenue, Cincinnati, OH 45267 (USA)

Abstract

Abstract. Serial studies were made of the membranes of the erythrocytes and the vesicles shed during storage of blood in polyvinyl chloride containers for 35 days in citrate-phosphate-dextrose-adenine anticoagulant. Special precautions were taken to eliminate artifacts created by contaminating leukocytes, platelets and red blood cell ghosts. A total of 15.6% of the cholesterol and 5.2% of the phospholipids of the membranes was lost with no gross change in the gel electrophoretic patterns. The quantity of vesicles found in the supernatant plasma increased during storage and their membranes were characterized by the absence of spectrin, ankyrin, and periodic acid Schiff bands 2 and 3. The ratio of lipids to protein in the vesicles increased as they accumulated perhaps reflecting a rearrangement of the erythrocyte membrane constituents during prolonged maintenance at 4°C.

Ancillary