This work was supported by the US Navy under Office of Naval Research Contract N00014–79-C-0168 with funds provided by the Naval Medical Research and Development Command. The opinions or assertions contained herein are those of the authors and are not to be construed as official or reflecting the views of the Navy Department or Naval Service at large.
Cryopreservation of Human Platelets Using 6% Dimethyl Sulfoxide and Storage at -80°
Effects of 2 Years of Frozen Storage at -80°C and Transportation in Dry Ice1
Version of Record online: 5 MAR 2009
© 1985 Blackwell Publishing Ltd
Volume 49, Issue 4, pages 245–258, October 1985
How to Cite
Melaragno, A.J., Carciero, R., Feingold, H., Talarico, L., Weintraub, L. and Valeri, C. R. (1985), Cryopreservation of Human Platelets Using 6% Dimethyl Sulfoxide and Storage at -80°. Vox Sanguinis, 49: 245–258. doi: 10.1111/j.1423-0410.1985.tb01119.x
- Issue online: 5 MAR 2009
- Version of Record online: 5 MAR 2009
- Recieved June 12, 1984 Revised January 28, 1985 Accepted March 4, 1985
Platelet studies were done in healthy male volunteers and in thrombocytopenic patients. Some of the platelets used in the study were isolated by mechanical apheresis using either the Haemonetics blood processor 30, the IBM blood processor 2997 or the Fenwal CS-3000 blood processor before freezing. Other platelets were isolated from individual units of whole blood and pooled before freezing. The platelets were frozen with a 6% cryoprotectant (DMSO) in a polyvinylchloride (PVC) plastic bag or a polyolefin plastic bag at -80°C in a mechanical freezer and stored for as long as 3 years. Some of the frozen platelets were transported in dry ice in polystyrene foam containers to determine whether they would be adversely affected by such treatment. Platelet recovery after freezing, thawing and washing was about 75%. In the healthy male volunteers, in vivo recovery of autologous platelets 1–2 h after transfusion was about 33%, and the life span was about 8 days. In the thrombocytopenic patients, in vivo recovery values were 50% of those from fresh platelets. The transfusion of previously frozen washed platelets reduced clinical bleeding in the thrombocytopenic patients with bleeding. There was no evidence of quality deterioration in platelets after storage at -80°C for at least 2 years, as determined from in vitro recovery and in vivo survival values, nor was there any adverse effect as a result of shipment of the frozen platelets in dry ice in polystyrene foam containers from one facility to another.