The efficacy of photochemical treatment with methylene blue and light for the reduction of Trypanosoma cruzi in infected plasma
Article first published online: 29 SEP 2006
Volume 91, Issue 4, pages 285–291, November 2006
How to Cite
Gironés, N., Bueno, J. L., Carrión, J., Fresno, M. and Castro, E. (2006), The efficacy of photochemical treatment with methylene blue and light for the reduction of Trypanosoma cruzi in infected plasma. Vox Sanguinis, 91: 285–291. doi: 10.1111/j.1423-0410.2006.00840.x
- Issue published online: 20 NOV 2006
- Article first published online: 29 SEP 2006
- Received: 9 March 2006, revised 3 August 2006, accepted 5 August 2006
- methylene blue;
Background and Objectives Chagas disease is a transfusion-transmitted infection. This study evaluates the efficacy of a methylene blue (MB) and light system for reducing the viability of Trypanosoma cruzi in plasma.
Materials and Methods Trypanosoma cruzi strains were spiked in plasma pools. Treatment arms included combined filtration, MB, light and freezing. Post-treatment parasite viability was assayed through in vitro cultures and in vivo inoculation in inducible nitric oxide synthase- and interferon-γ-receptor-deficient mice.
Results The filtration, MB and light combined treatment showed a log reduction of > 3·4 in in vitro cultures, and log reductions that ranged from > 4·9 to > 5·8 in deficient mice inoculated with different T. cruzi strains.
Conclusion The treatment of plasma units with the MB and light system reduces the T. cruzi burden and could be useful in preventing transfusion-transmitted Chagas disease.