Apheresis platelet concentrates contain platelet-derived and endothelial cell-derived microparticles
Article first published online: 4 AUG 2010
© 2010 The Author(s). Vox Sanguinis © 2010 International Society of Blood Transfusion
Volume 100, Issue 2, pages 179–186, February 2011
How to Cite
Rank, A., Nieuwland, R., Liebhardt, S., Iberer, M., Grützner, S., Toth, B. and Pihusch, R. (2011), Apheresis platelet concentrates contain platelet-derived and endothelial cell-derived microparticles. Vox Sanguinis, 100: 179–186. doi: 10.1111/j.1423-0410.2010.01385.x
- Issue published online: 11 JAN 2011
- Article first published online: 4 AUG 2010
- Received: 21 May 2010, revised 13 July 2010, accepted 16 July 2010, published online 4 August 2010
- endothelial cell;
- platelet concentrate
Background and Objectives Microparticles (MP) are membrane vesicles with thrombogenic and immunomodulatory properties. We determined MP subgroups from resting platelets, activated platelets and endothelial cells in donors and apheresis platelet concentrates (PC).
Material and Methods MP were double stained with annexin V and CD61 (platelet-derived MP; PMP), P-selectin or CD63 (MP from activated platelets) and CD144 plus E-selectin (endothelial cell-derived MP; EMP) and detected by flow cytometry in platelet donors (n = 36) and apheresis PC (n = 11; Trima™).
Results PC contained MP, mainly from resting platelets [93% (90–95)], and minor fractions of PMP from activated platelets [P-selectin+ or CD63+; 4·8% (3·2–7·7) and 2·6% (2·0–4·0)]. Compared to donors, levels of annexin V+ MP, PMP, P-selectin+ and CD63+ MP were 1·7-, 2·3-, 8·6- and 3·1-fold higher in PC (all P < 0·05). During storage (1–5 days), levels of annexin V+ MP and PMP did not increase, although small increases in the fraction of P-selectin+ or CD63+ MP occurred (both P < 0·05). PC also contained EMP, which were 2·6- to 3·7-fold enriched in PC compared to donors (P < 0·05).
Conclusions Transfusion of apheresis PC also results in transfusion of HLA-carrying PMP and EMP. This might counteract the aim of reducing transfused HLA load by leucodepletion. The increases in PMP exposing P-selectin or CD63 reflect mild platelet activation during storage. We conclude that in leucodepleted platelet apheresis using fluidized particle bed technology, MP are harvested mainly from the donor by apheresis. Improvement in apheresis technology might reduce MP load.