Association of CFU-Mk with total colony-forming units in thawed cord blood units
Article first published online: 22 JUN 2012
© 2012 The Author(s). Vox Sanguinis © 2012 International Society of Blood Transfusion
Volume 103, Issue 4, pages 331–336, November 2012
How to Cite
Eskola, M., Arvola, A., Juutistenaho, S., Aranko, K. and Kekomäki, R. (2012), Association of CFU-Mk with total colony-forming units in thawed cord blood units. Vox Sanguinis, 103: 331–336. doi: 10.1111/j.1423-0410.2012.01625.x
- Issue published online: 18 OCT 2012
- Article first published online: 22 JUN 2012
- Received: 12 January 2012, revised 1 March 2012, accepted 13 March 2012
- colony-forming unit;
- cord blood;
Background and Objectives Cord blood unit (CBU) total colony-forming unit (CFU) count both pre-cryo and post-thaw has been shown to be associated with platelet (PLT) engraftment. Pre-cryo CBUs show good growth of megakaryocytic CFUs (CFU-Mk); however, CFU-Mk have rarely been studied in post-thaw CBUs.
Materials and Methods Nucleated cells (NCs) from post-thaw CB were cultured in a collagen-based assay designed to support growth of CFU-Mk. To ensure accurate counting two independent investigators evaluated four culture chambers per sample for CFU-Mk growth. Post-thaw CFU and other cellular characteristics of the CBUs were enumerated independently and compared to CFU-Mk.
Results The post-thaw CBU total CFU count varied from 0·47 to 4·20 × 106 colonies (median, 0·99 × 106) and total CFU-Mk count from 0·11 to 0·70 × 106 colonies (median, 0·21 × 106). Total CFU-Mk count was closely associated with total CFU count (Spearman’s Rho = 0·86, P = 0·0072), haemoglobinized CFU (Rho = 0·86, P = 0·0072) and CFU-granulocyte/macrophage (CFU-GM; Rho = 0·81; P = 0·0154). Total CFU-Mk count also correlated with the post-thaw total CD34+ cell count (median, 2·55 × 106; range, 1·40–12·5 × 106; Rho = 0·83; P = 0·0154).
Conclusion CFU-Mk growth was associated with total CFU, haemoglobinized CFU, CFU-GM and CD34+ cells in thawed CBUs. This study confirms the preservation of CFU-Mk potential after CB cryopreservation.