Present address: Department of Immunology, Faculty of Medicine, Lorestan University of Medical Sciences (LUMS), Moalem Avenue, Khorram abad Lorestan, Iran.
International reference reagents to standardise blood group genotyping: evaluation of candidate preparations in an international collaborative study
Article first published online: 8 AUG 2012
© 2012 The Authors(s). Vox Sanguinis © 2012 International Society of Blood Transfusion
Volume 104, Issue 2, pages 144–152, February 2013
How to Cite
Boyle, J., Thorpe, S. J., Hawkins, J. R., Lockie, C., Fox, B., Matejtschuk, P., Halls, C., Metcalfe, P., Rigsby, P., Armstrong-Fisher, S., Varzi, A. M., Urbaniak, S. and Daniels, G. (2013), International reference reagents to standardise blood group genotyping: evaluation of candidate preparations in an international collaborative study. Vox Sanguinis, 104: 144–152. doi: 10.1111/j.1423-0410.2012.01641.x
- Issue published online: 17 JAN 2013
- Article first published online: 8 AUG 2012
- Received: 7 February 2012,revised 8 June 2012,accepted 25 June 2012,
- blood groups;
- molecular testing
Background and Objectives The aim of the study was to evaluate, in an international collaboration, four lyophilised genomic DNA preparations, selected from genotyped and phenotyped donors by the study organisers, for their suitability to standardise and control blood group genotyping procedures for common ancestral Caucasian and Black African alleles.
Materials and Methods Twenty-nine laboratories performed ‘blind’ testing of replicated ampoules of the candidate reference reagents, RBC1 (10/232), RBC4 (10/236), RBC5 (10/238) and RBC12 (10/234), using a range of genotyping procedures, most commonly classical PCR using allele or sequence specific primers.
Results The majority of laboratories reported blood group genotypes in accordance with those determined by the study organisers and the serological phenotypes. Despite an overall high level of accuracy in genotyping, the identified errors and inconsistencies, and the limited genotyping capabilities of many laboratories, confirmed the need for validated reference materials to control test procedures.
Conclusions The establishment of RBC1, RBC4, RBC5 and RBC12 as World Health Organization Reference Reagents will facilitate international standardisation of blood group genotyping and ensure that such tests are sufficiently sensitive and specific.