*Contributed equally to the paper.
Potential treatment of liver-related disorders with in vitro expanded human liver precursors
Article first published online: 9 MAY 2007
Volume 75, Issue 10, pages 928–938, December 2007
How to Cite
Yang, Y., Zheng, J., Zhou, X., Yang, Z., Tan, Y., Liu, A., Gao, X., Chang, Z. and Sheng, H. Z. (2007), Potential treatment of liver-related disorders with in vitro expanded human liver precursors. Differentiation, 75: 928–938. doi: 10.1111/j.1432-0436.2007.00184.x
- Issue published online: 9 MAY 2007
- Article first published online: 9 MAY 2007
- Received November 30, 2006; accepted in revised form March 21, 2007
- stem cell
Abstract Inherited deficiencies in critical components of metabolic pathways are the primary cause of many liver and lysosomal disorders, most of which are incurable. Stem cell transplantation may offer a new type of treatment for these diseases. We have isolated hepatocyte precursors from human fetal livers. These cells were highly proliferative in vitro in media with or without serum. Expanded hepatocyte precursors expressed endoderm and early hepatocyte markers. The precursors synthesized a large number of molecules related to human metabolic diseases and released some of them into the environment. In a homing test, these cells migrated preferentially into the liver. When transplanted into fetal sheep liver, they incorporated into the liver tissue and differentiated into hepatocytes. Transplantation of the liver precursors to α-l-iduronidase-deficient mice partially corrected the enzyme deficiency. Data from these studies suggest that in vitro expanded human liver precursor cells are a potential cell source for the treatment of liver- and lysosome-related disorders.