Get access

Potential treatment of liver-related disorders with in vitro expanded human liver precursors

Authors

  • Ying Yang,

    1. Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
    2. Center for Developmental Biology, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
      Tel: +86 21 55570017
      Fax: +86 21 55570017
    Search for more papers by this author
    • *Contributed equally to the paper.

  • Junke Zheng,

    1. Center for Developmental Biology, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
      Tel: +86 21 55570017
      Fax: +86 21 55570017
    Search for more papers by this author
    • *Contributed equally to the paper.

  • Xiaofei Zhou,

    1. Center for Developmental Biology, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
      Tel: +86 21 55570017
      Fax: +86 21 55570017
    Search for more papers by this author
  • Zhuqin Yang,

    1. Department of Obstetrics and Gynecology, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
    Search for more papers by this author
  • Yuedi Tan,

    1. Department of Obstetrics and Gynecology, The Central Hospital of Putou District, Shanghai, China
    Search for more papers by this author
  • Ailian Liu,

    1. Center for Developmental Biology, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
      Tel: +86 21 55570017
      Fax: +86 21 55570017
    Search for more papers by this author
  • Xiang Gao,

    1. Model Animal Research Center of Nanjing University, 12# Xuefu Road, Nanjing, Jiangsu, P.R. China, 210061
    Search for more papers by this author
  • Zai Chang,

    1. Model Animal Research Center of Nanjing University, 12# Xuefu Road, Nanjing, Jiangsu, P.R. China, 210061
    Search for more papers by this author
  • Hui Z. Sheng

    Corresponding author
    1. Center for Developmental Biology, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
      Tel: +86 21 55570017
      Fax: +86 21 55570017
      ✉ E-mail: hzsheng2003@yahoo.com
    Search for more papers by this author

✉ E-mail: hzsheng2003@yahoo.com

Abstract

Abstract Inherited deficiencies in critical components of metabolic pathways are the primary cause of many liver and lysosomal disorders, most of which are incurable. Stem cell transplantation may offer a new type of treatment for these diseases. We have isolated hepatocyte precursors from human fetal livers. These cells were highly proliferative in vitro in media with or without serum. Expanded hepatocyte precursors expressed endoderm and early hepatocyte markers. The precursors synthesized a large number of molecules related to human metabolic diseases and released some of them into the environment. In a homing test, these cells migrated preferentially into the liver. When transplanted into fetal sheep liver, they incorporated into the liver tissue and differentiated into hepatocytes. Transplantation of the liver precursors to α-l-iduronidase-deficient mice partially corrected the enzyme deficiency. Data from these studies suggest that in vitro expanded human liver precursor cells are a potential cell source for the treatment of liver- and lysosome-related disorders.

Get access to the full text of this article

Ancillary