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Table S1. PCR primers.

Fig. S1. Expression analysis of p38 MAPK in hES cells and early EBs by qRT-PCR. RNA was extracted from the undifferentiated hES culture (hES) and after overnight EB formation in SF (day 0). 3 independent wells were tested for each time point.

Fig. S2. EBs were grown in SF or END2-CM (CM) with or without the addition of 5 M SB203580 (+) during medium changes as indicated. The addition of the inhibitor after day 6 had no apparent effect on cardiomyogenesis.

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