Enhanced cardiomyogenesis of human embryonic stem cells by a small molecular inhibitor of p38 MAPK
Version of Record online: 16 NOV 2007
© 2007, ES Cell International Pte Ltd
Volume 76, Issue 4, pages 357–370, April 2008
How to Cite
Graichen, R., Xu, X., Braam, S. R., Balakrishnan, T., Norfiza, S., Sieh, S., Soo, S. Y., Tham, S. C., Mummery, C., Colman, A., Zweigerdt, R. and Davidson, B. P. (2008), Enhanced cardiomyogenesis of human embryonic stem cells by a small molecular inhibitor of p38 MAPK. Differentiation, 76: 357–370. doi: 10.1111/j.1432-0436.2007.00236.x
- Issue online: 20 NOV 2007
- Version of Record online: 16 NOV 2007
- Received May 21, 2007; accepted in revised form September 10, 2007
Table S1. PCR primers.
Fig. S1. Expression analysis of p38 MAPK in hES cells and early EBs by qRT-PCR. RNA was extracted from the undifferentiated hES culture (hES) and after overnight EB formation in SF (day 0). 3 independent wells were tested for each time point.
Fig. S2. EBs were grown in SF or END2-CM (CM) with or without the addition of 5 M SB203580 (+) during medium changes as indicated. The addition of the inhibitor after day 6 had no apparent effect on cardiomyogenesis.
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