Sloan-Kettering Institute for Cancer Research, New York, N. Y. 10021, U.S.A.
Synthesis and Use of O-Stearoyl Polysaccharides in Passive Hemagglutination and Hemolysis
Article first published online: 3 MAR 2005
European Journal of Biochemistry
Volume 1, Issue 1, pages 46–50, March 1967
How to Cite
Hämmerling, U. and Westphal, O. (1967), Synthesis and Use of O-Stearoyl Polysaccharides in Passive Hemagglutination and Hemolysis. European Journal of Biochemistry, 1: 46–50. doi: 10.1111/j.1432-1033.1967.tb00042.x
- Issue published online: 3 MAR 2005
- Article first published online: 3 MAR 2005
- (Received December 5, 1966)
Lipid-free polysaccharides generally are not attached to the surface of erythrocytes. In contrast O-stearoyl derivatives of polysaccharides show a high affinity for red blood cells. By reaction of polysaccharides with stearoyl chloride in dimethyl formamide as a solvent in the presence of pyridine, derivatives are obtained which exhibit marked erythrocyte-sensitizing activity. The chemical conditions for optimal sensitization of erythrocytes were investigated for a number of polysaccharides, e.g. starch, dextran, and several lipid-free bacterial cell wall polysaccharides. A content of about 5% by weight of O-stearoyl groups has been found most suitable for producing erythrocyte-sensitizing properties without modification of the serological specificity of the polysaccharide.
Sheep erythrocytes coated with O-stearoyl polysaccharide are specifically agglutinated by antipolysaccharide antibody (passive hemagglutination) or lysed when guinea pig complement is present at the same time (passive hemolysis).
Further applications of the method by using derivatives of O-stearoyl polysaccharides are mentioned.
- Dimethyl formamide
- phosphate-buffered saline
- 1Immunology, 1 (1964) 157., , , and ,
- 11Z. Naturforsch. 7b (1952) 148., , and ,
- 12D. Sc. Thesis, Albert-Ludwigs-Universität, Freiburg 1965.,
- 14Biochem. Z. 346 (1966) 346., , , and ,
- 151966) In preparation., et al. (
- 16Federation Proc. 19 (1960) 245.,
- 18Experimental Immunochemistry (edited by C. C.Thomas), Springfield, Illinois , 1961., and ,
- 21Bacteriol. Rev. 30 (1966) 193., , and ,
- 22Bacteriol. Proc. (1966) In press., and ,