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Abstract

  1. Top of page
  2. Abstract
  3. REFERENCES
  • 1
    The penetration of malate into Ascaris-muscle mitochondria and its metabolic conversion have been studied.
  • 2
    It is shown that Pi is required for the entry of malate into the mitochondria. In fact Pi activates: (1) the swelling of Ascaris-muscle mitochondria suspended in ammonium-malate; (2) the reduction by malate of mitochondrial nicotinamide nucleotides and flavoproteins; (3) the conversion of malate into other metabolites. Butylmalonate abolishes the activating effect of Pi on malate penetration into Ascaris-muscle mitochondria.
  • 3
    The activation by Pi of nicotinamide nucleotide reduction is followed at higher Pi concentrations by inhibition. 4,5,6,7-Tetrachloro-2-trifluoromethyl-benzimidazole inhibits the malatelinked reduction of nicotinamide nucleotides and flavoproteins.
  • 4
    Evidence is provided that in Ascaris-muscle two malate dehydrogenases are operative: the malic enzyme and the l-malate: NAD oxidoreductase.
Unusual Abbreviation
TTFB

4,5,6,7-tetrachloro-2-trifluoromethyl-benzimidazole

REFERENCES

  1. Top of page
  2. Abstract
  3. REFERENCES
  • 1
    Seidman, I., and Entner, N., J. Biol. Chem. 236 (1961) 915.
  • 2
    Lee, Y., and Chance, B., Biochem. Biophys. Res. Commun. 32 (1968) 547.
  • 3
    Chappell, J. B., and Haarhoff, K. N., In Biochemistry of Mitochondria (edited by E. C.Slater, Z.Kaniuga, and L.Woitczak), Academic Press, London 1967, p. 75.
  • 4
    Quagliariello, E., Papa, S., Meijer, A., and Tager, J. M., Symposium on Mitochondria-Structure and Function: 5th Meeting of the Federation of European Biochemical Societies, Prague 1968, Academic Press, in press.
  • 5
    Papa, S., Lofrumento, N. E., Loglisci, G., and Quagliariello, E., Biochim. Biophys. Acta, 189 (1969) 311.
  • 6
    Chance, B., and Hagihara, B., In Intracellular Respiration: Phosphorylating and non-Phosphorylating Oxidation Reactions (edited by E. C.Slater), Pergamon Press, London 1961, p. 3.
  • 6a.
    Rasmussen, H. N., unpublished observations.
  • 7
    Robinson, B. H., and Chappell, J. B., Biochem. Biophys. Res. Commun. 28 (1967) 249.
  • 8
    Weinbach, E. C., and Garbus, J., J. Biol. Chem. 241 (1966) 3708.
  • 9
    De Haan, E. J., and Tager, J. M., Abstr. Proc. Meet. Fed. Eur. Biochem. Soc., 3rd Warsaw 1966, Academic Press and P. W. N., London and Warsaw 1966, Academic.
  • 10
    Saz, H. J., and Hubbard, J. A., J. Biol. Chem. 225 (1957) 921.
  • 11
    Papa, S., Tager, J. M., and Quagliariello, E., In Regulatory Functions of Biological Membranes (edited by J.Jarnefelt), Elsevier Publishing Company, Amsterdam 1968, p. 264.
  • 12
    Kmetec, E., and Bueding, E., J. Biol. Chem. 236 (1961) 584.
  • 13
    Saz, H. J., and Vidrine, A., Jr., J. Biol. Chem. 234 (1959) 2001.
  • 14
    Von Brand, T., Biochemistry of Parasites, Academic Press, New York 1966, p. 115.