Localization of the Glycerol-Phosphate Dehydrogenase in the Outer Phase of the Mitochondrial Inner Membrane
Article first published online: 4 MAR 2005
European Journal of Biochemistry
Volume 13, Issue 2, pages 247–252, April 1970
How to Cite
Klingenberg, M. (1970), Localization of the Glycerol-Phosphate Dehydrogenase in the Outer Phase of the Mitochondrial Inner Membrane. European Journal of Biochemistry, 13: 247–252. doi: 10.1111/j.1432-1033.1970.tb00924.x
- Issue published online: 4 MAR 2005
- Article first published online: 4 MAR 2005
- (Received October 30/December 15, 1969)
Evidence for the localization of the mitochondrial glycerol-phosphate dehydrogenase in the outer phase of the inner membrane is presented.
- 1Glycerol-phosphate does not permeate through the inner membrane to the matrix space in a number of mitochondria which actively oxidize glycerol-phosphate.
- 2The impermeability of the inner membrane to ferricyanide is demonstrated and this forms a basis for elucidating membrane localization of carriers.
- 3In mitochondria from various tissues ferricyanide can interact directly with glycerolphosphate dehydrogenase but not with succinate dehydrogenase.
- 4The oxidation of glycerol-phosphate, in contrast to that of succinate, is insensitive to substrate depletion brought about by uncouplers.
- 5The results are interpreted to show that both the substrate and the acceptor-site of glycerol-phosphate dehydrogenase are exposed to the outer side of the inner membrane. Thus, for the operation of the glycerol-phosphate dehydrogenase, mitochondria do not require translocases for glycerol-phosphate and dihydroxyacetone-phosphate.