Localization of the Glycerol-Phosphate Dehydrogenase in the Outer Phase of the Mitochondrial Inner Membrane

Authors


Abstract

Evidence for the localization of the mitochondrial glycerol-phosphate dehydrogenase in the outer phase of the inner membrane is presented.

  • 1Glycerol-phosphate does not permeate through the inner membrane to the matrix space in a number of mitochondria which actively oxidize glycerol-phosphate.
  • 2The impermeability of the inner membrane to ferricyanide is demonstrated and this forms a basis for elucidating membrane localization of carriers.
  • 3In mitochondria from various tissues ferricyanide can interact directly with glycerolphosphate dehydrogenase but not with succinate dehydrogenase.
  • 4The oxidation of glycerol-phosphate, in contrast to that of succinate, is insensitive to substrate depletion brought about by uncouplers.
  • 5The results are interpreted to show that both the substrate and the acceptor-site of glycerol-phosphate dehydrogenase are exposed to the outer side of the inner membrane. Thus, for the operation of the glycerol-phosphate dehydrogenase, mitochondria do not require translocases for glycerol-phosphate and dihydroxyacetone-phosphate.
Unusual Abbreviation
FCCP

p-trifluoromethoxycarbonycyanidephenylhydrazone

Enzymes
 

Mitochondrial glycerol-1-phosphate dehydrogenase (EC 1.1.99.5)

 

cytosolic glycerol-1-phosphate dehydrogenase (EC 1.1.1.8)

 

glycerol kinase (EC 2.7.1.30)

 

succinate dehydrogenase (EC 1.3.99.1)

Ancillary