The mechanism of action of levansucrase of Bacillus subtilis was investigated by initial velocity measurements of reactions catalyzed by this enzyme and initial velocity of isotopic exchange at equilibrium. Comparison of experimental results with theoretical results derived from various postulated mechanisms, supports a “ping-pong” mechanism, involving the intermediate participation of a fructosyl-enzyme. Rate constants for each step of this sequential mechanism were estimated.
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- In kinetic equations and symbols the letters G
F, L, S and E0 represent glucose, fructose, levans, sucrose and total enzyme, respectively
Levansucrase or β-2,6-fructan: d-glucose 1-fructosyltransferase (EC 188.8.131.52)