Department of Biological Sciences, University of Warwick, Coventry, Great Britain CV4 7AL
Identification of Neighbouring Proteins in the Ribosomes of Escherichia coli
A Topographical Study with the Cross-Linking Reagent Dimethyl Suberimidate
Article first published online: 3 MAR 2005
European Journal of Biochemistry
Volume 42, Issue 1, pages 21–28, February 1974
How to Cite
Clegg, C. and Hayes, D. (1974), Identification of Neighbouring Proteins in the Ribosomes of Escherichia coli. European Journal of Biochemistry, 42: 21–28. doi: 10.1111/j.1432-1033.1974.tb03309.x
Nomenclature. The nomenclature of the ribosomal proteins follows that of Kaltschmidt and Wittmann; see Proc. Nat. Acad. Sci. U. S. A. (1970) 67, 1276–1282.
- Issue published online: 3 MAR 2005
- Article first published online: 3 MAR 2005
- (Received September 1, 1973)
Treatment of both the 30-S and 50-S ribosomal subunits of Escherichia coli with dimethyl suberimidate, a cross-linking agent specific for protein amino groups, resulted in the formation of new protein species detectable by acrylamide gel electrophoresis in sodium dodecylsulfate. These new species were shown to contain two or more ribosomal proteins linked together and, after cleaving the cross-links with ammonia, these constituents were identified by two-dimensional electrophoresis.