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Synthesis of Apamin, a Neurotoxic Peptide from Bee Venom

  1. Jurphaas VAN RIETSCHOTEN1,
  2. Claude GRANIER1,
  3. Hervé ROCHAT1,
  4. Francois MIRANDA1,
  5. Serge LISSITZKY2

Article first published online: 28 JUN 2008

DOI: 10.1111/j.1432-1033.1975.tb02204.x

Issue

European Journal of Biochemistry

European Journal of Biochemistry

Volume 56, Issue 1, pages 35–40, August 1975

Additional Information

How to Cite

VAN RIETSCHOTEN, J., GRANIER, C., ROCHAT, H., MIRANDA, F. and LISSITZKY, S. (1975), Synthesis of Apamin, a Neurotoxic Peptide from Bee Venom. European Journal of Biochemistry, 56: 35–40. doi: 10.1111/j.1432-1033.1975.tb02204.x

Author Information

  1. 1

    Laboratoire de Biochimie, Faculté de Médecine, Secteur Nord, Université d' Aix-Marseille, Boulevard Prerre-Dramard, F-13326 Marseille-Cedex-3, France

  2. 2

    Laboratoire de Biochimie Médicale, Faculté de Médecine, Université d'Aix-Marseille, 27 Boulevard Jean-Moulin, F-13385 Marseille-Cedex-4, France

Publication History

  1. Issue published online: 28 JUN 2008
  2. Article first published online: 28 JUN 2008
  3. (Received January 30/ March 28, 1975)

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The apamin sequence has been synthesized by the solid-phase procedure. The synthetic peptide showed the same physicochemical and chemical properties as natural apamin in the following tests: paper electrophoresis, amino acid analyses after acid and enzymatic hydrolyses, sequence analysis, electrophoreses after tryptic and chymotryptic digestions. Synthetic apamin was as active as natural apamin in the neurotoxic assay in mice (LD50, after subcutaneous injection, for the 20-g mouse: 58 μg).

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