A strong synergistic response was observed between the five endo-1,4-β-glucanases and the exo-1,4-β-glucanase obtained from culture solutions of the rot fungus Sporotrichum pulverulentum (formerly called Chrysosporium lignorum), when these enzymes were allowed to degrade de-waxed cotton and Avicel. No synergism was observed if Walseth cellulose, an acid-swollen cellulose, was used.
If de-waxed cotton was pretreated with endo-1,4-β-glucanases, the exo-1,4-β-glucanase enzyme released much more degradation products than from an untreated cotton. This strongly supports the theory that the endo-1,4-β-glucanases, acting randomly over the cellulose chain, go in first and open up chain ends where the exo-enzyme can act.
In an earlier paper [Eriksson, K.-E. & Pettersson, B. (1975) Eur. J. Biochem. 51, 193–206] physico-chemical differences between the endo-1,4-β-glucanases were demonstrated. Functional differences have now been found. The five endo-enzymes give rise to different degradation products from Avicel, show differences in preferred mode of attack on cellodextrins, and exhibit pronounced differences in synthetic activity.
The exo-1,4-β-glucanase released its products in the α-configuration.
A method for the separation of cellodextrins on a Biol-Sil A column is also described.
(Glc)3, (Glc)4, etc. represent the 1,4-β-oligoglucosides with 1, 2, 3, etc. glucose units
Endo-1,4-β-glucanase (EC 220.127.116.11)
exo-1,4-β-glucanase (EC 3.2.1.-)- β-glucosidase (EC 18.104.22.168)