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Phenylalanyl-tRNA and Seryl-tRNA from Synthetases Baker's Yeast
Substrate Specificity with Regard to ATP Analogs and Mechanism of the Aminoacylation Reaction
Article first published online: 28 JUN 2008
DOI: 10.1111/j.1432-1033.1976.tb10313.x
1742-4658/asset/cover.gif?v=1&s=fc98a9fc84ccc5e1b83463cabb40f397544364eb "European Journal of Biochemistry")
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How to Cite
FREIST, W., VON DER HAAR, F., SPRINZL, M. and CRAMER, F. (1976), Phenylalanyl-tRNA and Seryl-tRNA from Synthetases Baker's Yeast. European Journal of Biochemistry, 64: 389–393. doi: 10.1111/j.1432-1033.1976.tb10313.x
Publication History
- Issue published online: 28 JUN 2008
- Article first published online: 28 JUN 2008
- (Received June 12, 1975/January 19, 1976)
- Abstract
- Article
- References
- Cited By
Eighteen analogs of ATP have been tested in the aminoacylation reaction of phenylalanyl-tRNA and seryl-tRNA synthetases from baker's yeast. Four compounds. are substrates for phenylalanylLRNA syntiteiase, live for seryl-tRNA synthetase, one compound is an inhibitor for both enzymes; their Km and Ki and V values have been determined.
The substrate specificity shows that for the catalytic action of both enzymes with these substrates positions 6, 7, 8 and 9 of the purine moiety and positions 2′ and 3′ of the ribose moiety are important.