Immunochemical Evidence for the Difference between Coenzyme-B12-Dependent Diol Dehydratase and Glycerol Dehydratase

Authors

  • Tetsuo TORAYA,

    1. Laboratory of Industrial Biochemistry, Department of Industrial Chemistry, Faculty of Engineering, Kyoto University, Yoshida, Sakyo-Ku, Kyoto, Japan 606
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  • Saburo FUKUI

    1. Laboratory of Industrial Biochemistry, Department of Industrial Chemistry, Faculty of Engineering, Kyoto University, Yoshida, Sakyo-Ku, Kyoto, Japan 606
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  • This is Paper IX in the series on Coenzyme-B12-dependent diol dehydrase. The preceding paper in this series is [19].

Abstract

Klebsiella pneumoniae ATCC 25955 (formerly named Aerobacter aerogenes PZH 572, Warsaw), which is known to produce coenzyme-B12-dependent glycerol dehydratase when grown anaerobically in a glycerol medium, formed coenzyme-B12-dependent diol dehydratase in a 1.2-propanediol-containing medium. Both the diol dehydratase and the glycerol dehydratase produced by the organism catalyzed the conversion of glycerol, 1,2-propanediol and 1,2-ethanediol to the corresponding aldehydes and underwent concomitant inactivation during the catalysis of glycerol dehydration, as does the diol dehydratase of K. pneumoniae (A. aerogenes) ATCC 8724. However, the two enzymes were distinguishable from each other by the monovalent-cation-selectivity pattern and by substrate specificity; that is, glycerol dehydratase preferred glycerol to 1,2-propanediol as a substrate, whereas diol dehydratase preferred 1,2-propanediol to glycerol, as judged from initial velocity studies. Ouchterlony double-diffusion analysis and immunochemical titration with rabbit antiserum against diol dehydratase of K. pneumoniae ATCC 8724 established clearly that the diol dehydratase of K. pneumoniae ATCC 25955 is immunologically similar to that of K. pneumoniae ATCC 8724, while the glycerol dehydratase of the former is different from the diol dehydratase of both strains. Both the enzymes were found to be distributed in several bacteria of the family Enterobacteriaceae.

Abbreviation
Coenzyme B12

adenosylcobalamin or Coα-[α-(5,6-dimethylbenzimidazolyl)]-Coβ-adenosylcobamide

Enzyme
 

Diol dehydratase or d,l-1,2-propanediol hydro-lyase (EC 4.2.1.28)

 

Glycerol dehydratase or glycerol hydro-lyase (EC 4.2.1.30)

Ancillary