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Abstract

  1. Top of page
  2. Abstract
  3. REFERENCES
  • 1
    After hypotonic treatment spermatozoa have metabolic characteristics of mitochondria isolated from other cells. Ejaculated boar spermatozoa treated in this way can oxidise external NADH via both a lactate-pyruvate shuttle and a malate-aspartate cycle; this oxidation is coupled to the phosphorylation of ADP.
  • 2
    The dicarboxylate transport inhibitors butylmalonate, phenylsuccinate and bathophenanthroline sulphonate inhibit NADH oxidation dependent on added malate, glutamate and aspartate. α-Cyanocinnamate, a strong inhibitor of pyruvate transport, inhibits lactate-dependent NADH oxidation.
  • 3
    NADH oxidation dependent on malate, glutamate and aspartate is inhibited by uncoupling agents, but lactate-dependent NADH oxidation is stimulated.
  • 4
    Lactate-dependent NADH oxidation is inhibited by oxamate, an inhibitor of lactate dehydrogenase. Aminooxyacetate, an aminotransferase inhibitor, inhibits glutamate, malate and aspartate-dependent NADH oxidation.
  • 5
    Hypotonically-treated spermatozoa retain radioactivity after incubation with l-[U-14C]malate, [1.5-14C]citrate or [2-14C]malonate. Exchanges of retained radioactivity with various substrates indicate that dicarboxylate and tricarboxylate exchange carriers exist in the mitochondrial membrane.
Abbreviations
FCCP

carbonylcyanide p-trifluoromethoxy-phenylhydrazone

CCCP

carbonylcyanide m-chlorophenylhydrazone

Mops

3-(N-morpholino)propanesulphonic acid

Enzymes
 

Alcohol dehydrogenase (EC 1.1.1.1)

 

aspartate aminotransferase (EC 2.6.1.1)

 

FAD-linked glycerol-3-phosphate dehydrogenase (EC 1.1.99.5)

 

NAD-linked glycerol-3-phosphate dehydrogenase (EC 1.1.1.8)

 

lactate dehydrogenase (EC 1.1.1.27)

 

malate dehydrogenase (EC 1.1.1.37)

REFERENCES

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  2. Abstract
  3. REFERENCES
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