A Rare Genetically Determined Variant of Pseudocholinesterase in two German Families with High Plasma Enzyme Activity

Authors

  • Axel DELBRÜCK,

    1. Abteilung II, Institut für Klinische Chemie, Medizinische Hochschule Hannover, Zentrallabor am Krankenhaus Oststadt, Podbielskistraße 380, D-3000 Hannover 51, Federal Republic of Germany
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  • Eberhard HENKEL

    1. Abteilung II, Institut für Klinische Chemie, Medizinische Hochschule Hannover, Zentrallabor am Krankenhaus Oststadt, Podbielskistraße 380, D-3000 Hannover 51, Federal Republic of Germany
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  • Respectfully dedicated to Dr Fritz Lipmann, New York, on the occasion of his 80th birthday.

Abstract

Activity of pseudocholinesterase (acylcholine-acyl-hydrolase) elevated up to four times has been detected in sera of members of two German families. The catalytic concentrations of the pseudocholinesterase of the afflicted members of both families (male and female) varied between 4800 U/l and 10200 U/l (acetylthiocholine iodide substrate). The pseudocholinesterase of the propositi exhibits isoenzyme separation patterns in polyacrylamide electrophoresis as well as in electrofocussing which are different from those of pseudocholinesterase from normal persons. No differences could be seen as regards the Km of substrates or the inhibition by dibucaine, fluoride or succinyldiocholine.

Enzyme
 

Pseudocholinesterase or acylcholine acylhydrolase (EC 3.1.1.8)

Ancillary